Phytochemical analysis, antioxidant, antibacterial, and cytotoxic activities of leaves and roots of Rubus hyrcanus Juz.

Abstract Rubus hyrcanus Juz. (Rosaceae), known as Caspian blackberry, is wildly distributed around the Sea. This study focused on antioxidant, cytotoxic, and antibacterial activities of total extracts different fractions from roots leaves this species. The phenolics flavonoid contents were also evaluated. Finally, phenolic profiles selected determined using HPLC–DAD LC–MS/MS. results indicated that content (TPC) root extract (RTE) was 3.5 times (340.4 102.7 mg GAE/g, respectively). TPC three ranged 226.6 to 392.9 while in fractions, it between 68.3 101.8 GAE/g. had higher flavonoids than (70.5 8.9 QE/g, methanol both parts highest amounts flavonoids. fraction (RMF) best antioxidant effect DPPH radical scavenging assay (IC 50 : 9.16 μg ml −1 ) capacity test (1010.5 ɑTE/g). RMF RTE potent against Bacillus subtilis Staphylococcus aureus (MIC 1.5 ). In MTT assay, ethyl acetate exhibited cytotoxicity 247 227 , respectively) selectivity indexes (4.73 5.31, Phytochemical analysis revealed presence gallic acid, p-coumaric chlorogenic acid fraction, fraction.